Using T cell lymphokines of hyperimmunized chickens with Salmonella pullorum to enhance immune response of layer hens against avian influenza

The current study used immune lymphokines from chickens that had been hyperimmunized with Salmonella pullorum to improve the resistance of layer hens at production stage AIV-type (H5N1) infected (SP). Two groups (each consisting 25 pullets) were treated; first group received three doses SP vaccination 12, 14, and 16 weeks; second no was as a control group. At 18 weeks, T cells released (S-ILK), whereas produced non-immune (N-ILK). following procedures then on total 100-layer (ISSA brown), divided into four each. G1: S-ILK treatment H5N1 challenge. G2: N-ILK G3: Untreated challenged (H5N1). G4: untreated or unchallenged. Blood samples obtained 31, 32, 33 weeks age assess (IgG, IFN-γ). Additionally, lung tracheal tissues viral load influenza RNA copies (7 14) days experiment's findings indicated highest mean (IgG IFN-γ) titers lowest mortality other groups. test showed G2 G3 number significantly lower egg than G1, which kept normal level. conclusion shows using SILK gives higher level homogeneous protection against AIV without any dangers during production.